November 15, 2023


USDA-ARS researchers reduce classification of ASF strains to six genotypes




The US Department of Agriculture-Agricultural Research Service (USDA-ARS) has announced that researchers have reclassified the number of African swine fever (ASF) virus strains from 25 to only six unique genotypes.


This scientific innovation may help redefine how ASF researchers across the globe classify ASF virus (ASFV) isolates and may make it easier for scientists to develop vaccines that match the different strains circulating in ASF endemic areas across the globe.


The work is published in the journal Viruses.


"Previously, 25 different virus genotypes were identified across the globe," said senior ARS scientist Douglas Gladue. "Our research team recently re-evaluated all the publicly available virus DNA sequence and found that the majority of genotypes (genetic makeup) originally identified as novel were not correctly identified nor compared to already existing ASFV virus genotypes. Based on this analysis, there are actually fewer unique genotypes than the ASF research community believed, and that means that there is less diversity of ASFV affecting communities across the globe. This information is important as it may reduce the number of vaccines previously thought to be needed to protect against all ASFV genotypes."


Accurate classification of viruses is paramount for epidemiological investigations and the development of cost-effective countermeasures, including designing vaccines for unique strains of ASFV.


This large-scale effort involved ARS researchers re-analysing over 12,000 historical and current virus isolates that were produced from ASFV labs worldwide. The effort was made possible using the computing power of SciNet, which is ARS's supercomputer cluster for solving agricultural big data problems.


Although the ASFV virus is causing profound economic losses to the global swine industry, there have not been any outbreaks in the United States.


The research includes research and development input from the National Bio and Agro-Defense Facility, the Canadian Food and Inspection Agency, and Makerere University in Uganda.