Roles of fibre fermentation in monogastric animals: 2. Training microbiome to enhance fermentation of the major cereal NSPs
Results clearly demonstrated that gas production was significantly higher (up to 28%) in caecal microflora pre-adapted to a xylanase diet. This finding suggest that intestinal microflora can be trained to maximize fibre fermentation through supplementation of xylanase. Provision of xylo-oligosaccharides through action of xylanase may have upregulated metabolism of XOS utilising microbiota which increased gas production.
Figure 1. In vitro gas and butyric acid productions when cecal flora from broilers fed either Control diet or xylanase supplemented diet were used as inocula with various substrates. Broiler chickens were fed a wheat-soy diet which contained a xylanase (Econase XT, AB Vista, UK) at 0 or 16,000 BXU/kg for 35 days of age (Bedford and Apajalahiti, 2018).
Table 1. Sum of short chain xylo-oligosaccharide (X2-X5, g/100g) production after in vitro simulation of gizzard and small intestinal digestion in different wheat samples without and with xylanase (Econase XT) supplementation (Morgan et al., 2019).
Subsequent analysis on the pattern of volatile fatty acid production in Bedford and Apajalahti’s study showed that the caecal microflora adapted to a xylanase supplemented diet produced proportionally less acetate, propionate and higher butyrate. Increasing production of short chain XOS and butyric acid production with supplementation of xylanase has implications for intestinal health and energy extraction from the diet in older animals as microbial adaptation to altered substrate through supplementation of xylanase generally takes time (Masey-O’Neill et al., 2014). The burning topic is then whether intestinal microbiota can be trained to reduce the adaptation period and get most of the benefit via improving energy extraction and intestinal health from younger ages. This topic will be discussed in the next issue with in vivo responses from poultry and pig studies.
For more of the article, please click here.
Article made possible through the contribution of Jae Cheol Kim and AB Vista